abstract：:Despite reports that Tris [tris (hydroxymethyl)aminomethane] affects platelets, it is often used to buffer suspending media. Human or rabbit platelets were washed and resuspended in Tyrode solution containing apyrase and 0.35% albumin. Addition of 15 mM Tris partially inhibited primary aggregation induced by 10 microM...

journal_title：Thrombosis and haemostasis

authors： Packham MA,Guccione MA,Nina M,Kinlough-Rathbone RL,Mustard JF

更新日期：1984-04-30 00:00:00

abstract：:The strong effect of protein adsorption on blood and tissue compatibility is well known. Little is presently known about the mechanisms which control the composition of the adsorbed protein layer which forms upon exposure of surfaces to mixtures of proteins. Reexamination of the ability of hemoglobin to inhibit the ad...

journal_title：Thrombosis and haemostasis

authors： Horbett TA

更新日期：1984-04-30 00:00:00

abstract：:The functional activity and active site of plasmin in full-term newborns have been studied and compared to those in adults in order to investigate the nature of the abnormality found in newborn plasminogen described in a previous paper. The functional activity of newborn plasminogen measured on chromogenic substrate w...

journal_title：Thrombosis and haemostasis

authors： Benavent A,Estellés A,Aznar J,Martinez-Sales V,Gilabert J,Fornas E

更新日期：1984-02-28 00:00:00

abstract：:The fibrin-mediated enhancement of the activation of plasminogen by tissue-type plasminogen activator observed with normal fibrin, is strongly decreased with fibrin Dusard, although the binding of tissue-type plasminogen activator to this fibrin is normal. This impaired fibrin-mediated plasminogen activation is most l...

journal_title：Thrombosis and haemostasis

authors： Lijnen HR,Soria J,Soria C,Collen D,Caen JP

更新日期：1984-02-28 00:00:00

abstract：:Mouse mesothelium has been examined as a surface for supporting blood flow. We have examined ten pieces of intact mesothelium and ten pieces of damaged mesothelium following 10 min exposure to flowing blood in a Baumgartner chamber. Scanning electron microscopy of the intact specimens demonstrated no adhering blood pl...

journal_title：Thrombosis and haemostasis

authors： Clarke JM,Pittilo RM,Machin SJ,Woolf N

更新日期：1984-02-28 00:00:00

abstract：:We tested the response of the fibrinolytic activity and factor VIII-antigen levels to infusion of DDAVP in healthy volunteers and we studied the influence of propranolol and aspirin on this response. After DDAVP, 0.4 microgram/kg in 10 min i.v., the fibrinolytic activity of redissolved euglobulins rose from 179 to 452...

journal_title：Thrombosis and haemostasis

authors： Brommer EJ,Derkx FH,Barrett-Bergshoeff MM,Schalekamp MA

更新日期：1984-02-28 00:00:00

abstract：:We studied human platelet aggregation and beta-TG/PF4 release induced by heparin and related GAGs in vitro both in normal PRP and in PRP after aspirin. In our experimental conditions, heparin and related GAGs always caused PF4 release in vitro from normal platelets, whether or not there was measurable platelet aggrega...

journal_title：Thrombosis and haemostasis

authors： Cofrancesco E,Colombi M,Cristoforetti G,Pogliani EM

更新日期：1984-02-28 00:00:00

abstract：:Venous oxygen tension (pO2) was measured in discrete samples of blood obtained through the femoral vein of cardiac catheterisation patients before, during and after application of sustained external graduated pressure in the form of compression stockings (T. E. D. Kendall). There was a significant reduction (p less th...

journal_title：Thrombosis and haemostasis

authors： Okoye GC,Evans JH,Beattie J,Lowe GD,Lorimer AR,Forbes CD

更新日期：1984-02-28 00:00:00

abstract：:The effect of negatively-charged polymers, used in some artificial devices, on plasma clotting and kinin systems was studied in vitro using polyelectrolyte complexes. Contact activation was observed as an immediate, transient and surface-dependent phenomenon. After incubation of the plasma with the polymer a small dec...

journal_title：Thrombosis and haemostasis

authors： Boffa MC,Dreyer B,Pusineri C

更新日期：1984-02-28 00:00:00

abstract：:Forty-two patients with total occlusion of a coronary vessel were treated with intracoronary fibrinolytic agents. Four therapeutic protocols were compared: group I received streptokinase (SK) as a continuous infusion; group II and III received SK as a bolus at different doses and group IV received lysplasminogen (Pg) ...

journal_title：Thrombosis and haemostasis

authors： de Prost D,Guerot C,Laffay N,Horellou MH,Samama M

更新日期：1983-12-30 00:00:00

abstract：:There are conflicting views on the effects of age, gene source and familial severity on levels of factor VIII in carriers of haemophilia. Different workers have found that factor VIII increases with age, is higher in paternal carriers, and is higher in carriers from families with more severe haemophilia. Other workers...

journal_title：Thrombosis and haemostasis

authors： Duncan BM,Tunbridge LJ,Lloyd JV

更新日期：1983-10-31 00:00:00

abstract：:The prothrombin time (PT) test is used to monitor oral anticoagulant therapy. Contact activation of patient blood samples in vitro may shorten the PT and thus lead to underestimation of the degree of anticoagulation. Commercial evacuated tubes for blood collection have been reported to induce shortening of the PT. The...

journal_title：Thrombosis and haemostasis

authors： van den Besselaar AM,van Halem-Visser LP,Loeliger EA

更新日期：1983-10-31 00:00:00

abstract：:Thirty-three patients with acute iliofemoral thrombosis were randomly assigned to three treatment groups in a pilot dose-ranging study of thrombolytic therapy in deep vein thrombosis. One group received tissue culture urokinase in a dose of 2,200 I.U./kg/hr, and a second group in a dose of 1,100 I.U./kg/hr following a...

journal_title：Thrombosis and haemostasis

authors： van de Loo JC,Kriessmann A,Trübestein G,Knoch K,de Swart CA,Asbeck F,Marbet GA,Schmitt HE,Sewell AF,Duckert F

更新日期：1983-10-31 00:00:00

abstract：:An international collaborative study involving seven laboratories was undertaken to assess the suitability of a freeze-dried preparation of human plasmin to replace the current International Reference Preparation (IRP) for plasmin. Chromogenic and fibrinolytic assays were used by all participating laboratories to asse...

journal_title：Thrombosis and haemostasis

authors： Gaffney PJ,Mussett MV

更新日期：1983-10-31 00:00:00

abstract：:Thrombin-induced platelet release reaction examined with secretion of calcium and N-acetylglucosaminidase was significantly enhanced in the platelets from reserpine-treated rabbits as compared with the control. On the other hand, 32P-incorporation into phosphatidic acid was suppressed in the reserpinized platelets in ...

journal_title：Thrombosis and haemostasis

authors： Watanabe Y,Soda M,Fukamachi N,Kobayashi B

更新日期：1983-08-30 00:00:00

abstract：:An important physiological anti-thrombotic function has been suggested for plasma fibronectin and the reticuloendothelial system. The current study evaluated the effects of specific immunological fibronectin depletion and fibronectin supplementation upon resistance to infusion of thrombin and ADP in terms of mortality...

journal_title：Thrombosis and haemostasis

authors： Kaplan JE,Snedeker PW,Baum SH,Moon DG,Minnear FL

更新日期：1983-06-28 00:00:00

abstract：:It was found that the effect of heparin on the amidase activity of urokinase (E C 3.4.21.31), plasmin (E C 3.4.21.7) and trypsin (E C 3.4.21.4) depended on the substrate used. No effect of heparin on the amidase activity of urokinase and trypsin was observed when Pyro Glu-Gly-Arg-p-nitroanilide (S-2444) and alpha-N-ac...

journal_title：Thrombosis and haemostasis

authors： Yomtova VM,Stambolieva NA,Blagoev BM

更新日期：1983-06-28 00:00:00

abstract：:The clearance of radiolabeled human fibrinogen fragments X and Y was studied in the mouse model. Fragment X cleared rapidly from the circulation with less than 10% of the ligand remaining in the circulation at 4 hr. The clearance of fragment Y was somewhat slower, but was identical to the rate of clearance of fragment...

journal_title：Thrombosis and haemostasis

authors： Pasqua JJ,Pizzo SV

更新日期：1983-04-28 00:00:00

abstract：:A double antibody competition radioimmunoassay was developed that allowed to detect specifically as little as 15 ng antithrombin III (AT III) per ml of the assayed material. In normal plasma examined by this assay, AT III concentration averaged 199 +/- 21 micrograms/ml. Complexes of AT III with thrombin or factor Xa c...

journal_title：Thrombosis and haemostasis

authors： Marciniak E,Gora-Maslak G

更新日期：1983-02-28 00:00:00

abstract：:Accumulation of the newly formed 14C-cyclic adenosine 3',5'-monophosphate (cyclic AMP) was found in the P1 (1.0) fraction, i.e. a platelet plasma membrane fraction which was obtained from 14C-adenine-labeled platelets. On the other hand, total cyclic AMP as determined simultaneously was located mainly in the platelet ...

journal_title：Thrombosis and haemostasis

authors： Hashimoto S

更新日期：1983-02-28 00:00:00

abstract：:In order to assess the value of I.P.G. for the diagnosis of D.V.T. in general practice, an I.P.G. was carried out by a skilled technician in 255 consecutive patients with suspected D.V.T. at home. Ascending venography was carried out in 185 of these patients. In addition, blood for assay of AT III, platelet count, fib...

journal_title：Thrombosis and haemostasis

authors： Peters SH,Jonker JJ,de Boer AC,den Ottolander GJ

更新日期：1982-12-27 00:00:00

abstract：:The measurement of platelet deposition in human thrombi is essential for the evaluation of platelet-inhibitory drugs and prosthetic materials for use in patients. The rate of 111Indium-labelled platelet accumulation on Dacron arterial grafts was measured in 27 patients randomised to take either aspirin and dipyridamol...

journal_title：Thrombosis and haemostasis

authors： Norcott HC,Goldman M,Hawker RJ,Rafiqi EI,Drolc Z,McCollum CN

更新日期：1982-12-27 00:00:00

abstract：:The effects of etamsylate on human platelet aggregation and ATP release as well as on the arachidonate metabolism by the platelet have been studied. Etamsylate enhanced these platelet functions induced by arachidonic acid (AA), thromboxane A2, collagen and calcium ionophore A23187 but not those induced by ADP and epin...

journal_title：Thrombosis and haemostasis

authors： Okuma M,Takayama H,Sugiyama T,Sensaki S,Uchino H

更新日期：1982-12-27 00:00:00

abstract：:Acid-treated human plasma is a competitive inhibitor of the hydrolysis of D-Val-Leu-Lys-Nan (S-2251) by plasmin. The rate of hydrolysis is decreased to 50% by 750 fold diluted acidified normal plasma and by 60 fold diluted acidified alpha 2-antiplasmin depleted plasma (alpha 2-antiplasmin concentration less than 2%). ...

journal_title：Thrombosis and haemostasis

authors： Lijnen HR,Maes M,Castel M,Samama M,Collen D

更新日期：1982-12-27 00:00:00

abstract：:Following exposure of rats to graded doses of fresh cigarette smoke, the aortic endothelium examined by scanning electron microscopy regularly showed alterations. These essentially consisted of areas of blebbing and microvillus-like projections from the luminal surface, and the presence in the majority of cases, of mi...

journal_title：Thrombosis and haemostasis

authors： Pittilo RM,Mackie IJ,Rowles PM,Machin SJ,Woolf N

更新日期：1982-10-29 00:00:00

abstract：:Although there is considerable evidence that platelet activation is associated with polymerization of actin, it is not known whether there is some pre-existing F-actin even before activation. We have examined the state of actin polymerization in nonactivated platelets by deoxyribonuclease assay of G-actin and total ac...

journal_title：Thrombosis and haemostasis

authors： Davies GE,Palek J

更新日期：1982-10-29 00:00:00

abstract：:Hot water extracts of Mo-er (1 gm by 15 ml of water), an oriental food (Auricularia auricula), inhibit strongly both human and rat platelet ADP-induced aggregation. HPLC analysis of two varieties of Mo-er, A. auricula and A. polytricha (a black tree fungus), shows that they contain adenosine (Ado), 133 and 154 microgr...

journal_title：Thrombosis and haemostasis

authors： Agarwal KC,Russo FX,Parks RE Jr

更新日期：1982-10-29 00:00:00

abstract：:Sequential treatment of arterial occlusions of the leg with porcine plasmin and low dose streptokinase results in a strong systemic proteolysis as already seen in deep leg vein thrombosis. In 31 of 45 patients the blood flow through major arterial segments could be restored. Thrombolytic success is possible within the...

journal_title：Thrombosis and haemostasis

authors： Marbet GA,Eichlisberger R,Duckert F,da Silva MA,Biland L,Widmer LK,Ritz R,Schmitt HE

更新日期：1982-10-29 00:00:00

abstract：:The tail bleeding time (BT) in rats definitely varies according to the method applied. Of the various variables that may influence BT, we have evaluated the position (horizontal or vertical) of the tail, the environment (air or saline), the temperature (4 degrees, 23 degrees or 37 degrees C) and the type of anaesthesi...

journal_title：Thrombosis and haemostasis

authors： Dejana E,Villa S,de Gaetano G

更新日期：1982-08-24 00:00:00

abstract：:We studied the recovery pattern of platelet malondialdehyde (MDA) formation after a single intake of various doses of aspirin (ASA). In normal subjects, there were striking differences in the recovery pattern between large doses (1,000 or 500 mg) and small doses (100 or 50 mg) of oral ASA. The 2 day lag phase was pres...

journal_title：Thrombosis and haemostasis

authors： Takayama H,Okuma M,Uchino H

更新日期：1982-08-24 00:00:00

abstract：:In order to determine the molecular size of human F VIII as found in fresh plasma and as found in fresh-frozen plasma and in cryoprecipitate and to study the relationship of the factor-related properties, namely F VIII:C, F VIIR:Rcof and F VIIIR:Ag to F VIII, the factor's elution pattern on Sephacryl 1000 was investig...

journal_title：Thrombosis and haemostasis

authors： Vukovich TC

更新日期：1982-08-24 00:00:00

abstract：:Tissue thromboplastin apoprotein was partially purified from human brain. The apoprotein was recombined with mixed phospholipids to yield active thromboplastin. The recombined thromboplastin induced proteolytic activation of isolated human factor IX in the presence of factor VII and Ca2+. The clotting times of various...

journal_title：Thrombosis and haemostasis

authors： van den Besselaar AM,Ram IE,Alderkamp GH,Bertina RM

更新日期：1982-08-24 00:00:00

abstract：:In order to detect impaired synthesis of blood coagulation factors associated to consumption coagulopathy, a simultaneous evaluation of factor II-related antigen (II rAg) and of antithrombin III (AT III) was carried out in 16 patients affected with severe defibrination. An in vitro preliminary study on plasma and seru...

journal_title：Thrombosis and haemostasis

authors： Sié P,Letrenne E,Caranobe C,Genestal M,Cathala B,Boneu B

更新日期：1982-06-28 00:00:00

abstract：:Haemostatic parameters were assessed before insulin induced hypoglycaemia and 0, 1 and 2 hr after discontinuation of insulin infusion in 7 non-diabetics, aged 28 (22-31) years (mean and range), and 8 juvenile diabetics, aged 31 (27-35) years, with a mean duration of diabetes of 4 years. The patients were normoglycaemi...

journal_title：Thrombosis and haemostasis

authors： Dalsgaard-Nielsen J,Madsbad S,Hilsted J

更新日期：1982-06-28 00:00:00

abstract：:Human fibrinogen was subjected to limited proteolytic attack by thrombin, batroxobin or Agkistrodon contortrix thrombin-like enzyme, yielding desAB-, desA- or desB-fibrin monomers, respectively. Turbidity curves demonstrated that, with all three enzymes, the polymerization process was strongly accelerated by increasin...

journal_title：Thrombosis and haemostasis

authors： Furlan M,Rupp C,Beck EA,Svendsen L

更新日期：1982-04-30 00:00:00

abstract：:A preliminary clinical trial was conducted to determine the feasibility of achieving and regulating therapeutic anticoagulation with heparin given by continuous subcutaneous infusion. Five patients with deep venous thrombosis confirmed by impedance plethysmography and/or venography were studied. All patients received ...

journal_title：Thrombosis and haemostasis

authors： Parrilla N,Ansell J

更新日期：1982-02-26 00:00:00

abstract：:FPA level, fibrinogen turnover rate, and fibrinolytic activity were studied on 18 patients with malignant disease. It was found that the FPA levels were significantly elevated and were correlated with fibrinogen turnover rate (r = 0.74, p less than 0.001) and FDP (r - 0.58, p less than 0.02). Estimated FPA turnover ra...

journal_title：Thrombosis and haemostasis

authors： Yoda Y,Abe T

更新日期：1981-12-23 00:00:00

abstract：:Injection of human alpha 2-antiplasmin resulted in delayed elimination of fibrin from the lungs in rats with thrombin-induced intravascular coagulation. The amount of alpha 2-antiplasmin injected was chosen so as to simulate the posttraumatic increase of this inhibitor in man. By injecting trace amounts only of radiol...

journal_title：Thrombosis and haemostasis

authors： Carlin G,Einarsson M,Saldeen T

更新日期：1981-12-23 00:00:00

abstract：:Thrombin incubated with 2,3-diphosphoglycerate (150 nmol 2,3-DPG/1 NIH thrombin unit) lost up to 70% of its clotting activity, whereas the esterase activity remained unchanged. No fibrinopeptide release by thrombin was observed in the presence of 2,3-DPG. The fibrin polymerization was normal. By chromatography on Ambe...

journal_title：Thrombosis and haemostasis

authors： Del Principe D,Strappini PM,Del Vecchio S,Menichelli A,Biancini G,Cosmi EV,Bastianon V,D'Arcangelo C

更新日期：1981-10-01 00:00:00

abstract：:Samples taken at various stages of preparation of intermediate purity factor VIII concentrate were assayed for factor VIII coagulant activity (VIII:C), factor VIII coagulant antigen (VIIIC:Ag) and factor VIII related antigen (VIIIR:Ag). The antigen results were used to assess mechanical loss during fractionation as th...

journal_title：Thrombosis and haemostasis

authors： Prowse CV,Griffin B,Pepper DS,Dickson AJ,McQuillan TA,Dickson IH,Foster PR

更新日期：1981-10-01 00:00:00